KU560-R-4 is is the rigid derivative of the NHS-ester significantly restricting the local motion in the dye and linker.
KU560 is robust dye that can be used in unique polarisation based experiments as the fluorescence of the free dye and dye-biomolecule conjugates exceed 15 ns.
KU560 is ideal dyes for epifluorescence imaging as it does not bleach under unfocused illumination.
|
λ(abs) |
560 nm |
|---|---|
|
ε(max) |
16,800 M -1cm-1 |
|
λ(fl) |
590 nm |
|
Φ(fl) |
>50 % |
|
τ(fl) |
~20 ns |
|
CF260 = ε(260)/ε(max) |
2.98 |
|
CF280 = ε(280)/ε(max) |
1.41 |
