KU530-R-4 is the rigid derivative of the NHS-ester completely removing local motion in the dye and linker.
KU530 is robust dye that can be used in unique polarisation based experiments as the fluorescence of the free dye and dye-biomolecule conjugates exceed 15 ns.
KU530 is ideal dyes for epifluorescence imaging as it does not bleach under unfocused illumination.
|
λ(abs) |
530 nm |
|---|---|
|
ε(max) |
9,800 M -1cm-1 |
|
λ(fl) |
560 nm |
|
Φ(fl) |
>60 % |
|
τ(fl) |
~24 ns |
|
CF260 = ε(260)/ε(max) |
4.53 |
|
CF280 = ε(280)/ε(max) |
1.27 |
